DogBAC is a BAC library which is screened by PCR. It comprises 212160 clones with insert length varying from 70 kb to 170 kb. DogBAC contains about 93% of the canine genome with a 4-fold coverage. The vector is pBeloBAC11 (EMBL U51113). The clones are grown in LB (Difco) with chloramphenicol (25 mg/ml).

Under the following conditions you can obtain clones:

1. Clones of DogBAC are only made accessible within a collaboration.
   
2. The first peer-reviewed full length publication making use of a DogBAC clone is co-authored by G. Dolf and C. Schelling.
   
3. Subsequent publications refer to Schelling C, Schläpfer J, Billaut A, Guziewicz K, Gmür A, Katmann I, Pineroli B, Colomb B, Rickli O, Wittwer C, Piasecka A, Dolf G, 2002. Construction of a canine artificial chromosome library for screening with PCR. Journal of Animal Breeding and Genetics 119, 400-401.
4. All information derived from DogBAC clones must be made available to our public BAC database at the latest immediately after publication. Results from DNA sequencing must be submitted to EMBL or Genbank.
5. We do not grant exclusive rights to clones. Other laboratories may obtain the same clones. We reserve the right to use all clones for our own purposes. In case we or a second laboratory needs the same clone as the first laboratory, it is agreed that investigations by us or a second laboratory must not interfere with the investigation of the first laboratory.
6. DogBAC clones or their derivatives may not be passed on to third parties without our consent.

Under the following procedure you can obtain clones:

1. You have to provide primer sequences or primer pairs with the appropriate labelling (IRD800) together with the PCR conditions and the expected PCR product length. A picture demonstrating the successful amplification of canine DNA would be appreciated.
2. Should PCR conditions deviate from standard conditions the PCR buffer must be provided.
3. We will screen the DogBAC library and isolate positive clones. Up to three positive clones will be shipped as stab cultures. Should more than three positive clones be identified they will be provided as needed.
4. We will keep a glycerol stock of each clone shipped. After conclusion of a project we may discard the glycerol stocks.

Special arrangements can be negotiated.